Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

Effect of the eRF1 (sup45-M48I) mutation with impaired decoding ability on hc tryptophan or cysteine nc-tRNA incorporation at UGA-N termination tetranucleotides. (A) The L2327 was transformed with either empty vector (EV) or hc tW(CCA)G1 and the resulting transformants were grown and processed for stop codon readthrough measurements as described in Figure 1. (B) Essentially the same as in A, except that hc tC(GCA)P1 was used in place of hc tW(CCA)G1.