FIGURE 3.
Effect of the miscoding agent paromomycin on hc tryptophan or cysteine nc-tRNA incorporation at UGA-N termination tetranucleotides. (A) The PBH140 derivative bearing TIF35 wt was transformed with either empty vector (EV) or hc tW(CCA)G1, and the resulting transformants were grown in SD with 200 µg/mL paromomycin for 6 h and processed for stop codon readthrough measurements as described in Figure 1. (B) Essentially the same as in A, except that hc tC(GCA)P1 was used in place of hc tW(CCA)G1.
