Oligoadenylation of 3′ decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells

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FIGURE 1.
FIGURE 1.

Library preparation for Illumina sequencing. S2 cells expressing the inducible Hsp70 reporter were treated with dsRNA as indicated in the text. Reporter gene transcription was induced with CuSO4 for 1 h, and total RNA was isolated. After barcoded linker addition, PCR was performed as indicated. A size selection (bracket) was performed for products of 200–300 nt, which were subsequently analyzed by Illumina sequencing.

This Article

  1. Published in Advance January 19, 2016, doi: 10.1261/rna.053942.115 RNA 22: 428-442

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