A DEAD-box RNA helicase promotes thermodynamic equilibration of kinetically trapped RNA structures in vivo

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FIGURE 3.
FIGURE 3.

Mss116p did not affect chimeric mRNA stability or self-cleavage activity nonspecifically in yeast. (A) Secondary structure of HP210, which does not contain a complementary insert with the potential to form an alternative helix. Chimeric HP210 mRNAs were coexpressed in yeast with (B) active Mss116p (Δmts), (C) catalytically inactive Mss116p (ΔmtsE268Q), or (D) empty vector. Plots display results from a representative pair of experiments with functional (▪) and mutationally inactivated (□) chimeric HP210 mRNAs. Reported values represent the mean and standard deviation obtained from two or more pairs of experiments.

This Article

  1. Published in Advance January 12, 2016, doi: 10.1261/rna.055178.115 RNA 22: 416-427

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