FIGURE 4.
Effect of polyethylene glycol linker on the function properties of DbpA. (A) The RNA-polyethylene glycol chimera construct used for these experiments. The nine-atom ethylene glycol linker spans three RNA bases. (B) Helicase activity of DbpA in the presence of RNA-polyethylene glycol chimera. These data represent one experiment performed at 600 nM DbpA. The observed rate constants of unwinding average values obtained by multiple independent experiments and various DbpA concentrations are shown in Table 1.
