FIGURE 3.
Effect of physical distance between the double-helix substrate and helix 92 on the DbpA protein helicase activity. (A) The model RNA–DNA chimera used for this experiment. (B) Helicase activity of the DbpA protein in the presence of longer RNA–DNA chimera. These data represent one experiment performed at 600 nM DbpA. The observed rate constants of unwinding average values obtained by multiple independent experiments and various DbpA concentrations are shown in Table 1.
