FIGURE 6.
PNPase enhances Hfq-binding of MicA sRNA. (A) Northern blot analysis of RNAs coimmunoprecipitated with Hfq. Hfq was immunoprecipitated from extracts of a wild-type strain (KR10000; WT), hfq deletion strain (NRD1094; Δhfq), or pnp deletion strain (NRD1369; Δpnp) prepared from late exponential phase cultures using anti-Hfq antibody bound to protein A-sepharose resin. For Northern blots, the ratio of the amount of RNA from the input and elution fractions loaded was 16:1, and for Western blots, the ratio of protein samples loaded was 1:18. (B) Fold enrichment of a given RNA upon immunoprecipitation with Hfq was calculated as described in Figure 1B.
