FIGURE 5.
Hfq protects some sRNAs from PNPase degradation. (A) In vitro assay on sRNA degradation by PNPase in the presence and absence of Hfq. RyhB, GcvB, and CyaR were incubated with PNPase or PNPase and Hfq in the presence of 2 mM phosphate. Reactions were quantified and standard deviations were estimated from triplicates (lower panel). (B) In vitro assay of sRNA degradation in the presence of tungstate. Assays were performed as in A, except tungstate was added at a final concentration of 2 or 10 mM.
