FIGURE 2.
Association of PNPase with RyhB in vivo is affected by active site residues S437, S438, and S439 and the S1 domain. (A) Flag-tagged PNPase, or derived variants lacking the S1 domain or having three active site residues mutated (S437A S438A S439A) were used to coprecipitate sRNAs, which were analyzed by Northern blot. For Western blots, an equal volume of protein samples from input and elution were loaded, and for Northern blots, the ratio of input to elution loaded was 1:162. (B) Fold enrichment of RNA upon immunoprecipitation was calculated as in Figure 1.
