FIGURE 4.
Sequencing of pre-tRNAUAUIle forms document 3′ end processing defects under stress conditions. (A) Outline of the strategy for linker ligation and sequencing of pre-tRNA 3′ ends. (B) Section of pre-tRNAUAUIle subjected to sequencing. (C) Summary of results obtained for pre-tRNAUAUIle sequencing of RNAs isolated from cells grown in YPD 30°C or shifted for 2 h to YPGly at 37°C or 39°C. Percent of pre-tRNAUAUIle molecules with 3′ processed or 3′ extended ends was calculated from the total number of sequenced clones that corresponded to genomic sequence and/or additional modification (A or CCA).
