Fingerprinting the junctions of RNA structure by an open-paddlewheel diruthenium compound

(A) HCV IRES reactivity toward OPW-Ru and NMIA as a function of the nucleotide position. RNA incubated with OPW-Ru (10 µM) for 15 min at 37°C RNA reactivity is colored according to the scale shown on the right. Values correspond to the mean ± SD of three independent experiments. (B) OPW-Ru reactive positions on the HCV IRES secondary structure, compared to NMIA reactivity (C). The inset in B highlights the position of reactive residues on the flexible regions (orange dots) of the RNA structure model adapted from Perard et al. (2013).