FIGURE 6.
Summary and validation of the AP-MS results for SL-let-7a-1 and SL-let-7g. (A) The overlap of identified proteins in the SL-let-7a-1 and SL-let-7g ARiBo pull-downs is shown using Venn diagrams. The total proteins identified were taken from proteomic analysis using the mock and dlet-7g controls. The proteins that likely bind the duplex region are those identified using the mock control but not the dlet-7g control. (B) Validation of the AP-MS results by Western blots of the elution fractions (50% of E1) from the SL-let-7a-1, SL-let-7g, and mock pull-downs.
