ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry

ARiBo pull-down with an NT2 WCE. (A) Aliquots of the elution fractions (90% of E1) from the RNA pull-downs of the ARiBo tag only (mock) and SL-let-7g-ARiBo (SL-let-7g) with an NT2 WCE analyzed on a Coomassie-stained SDS-PAGE gel. (B) Western blot with an anti-Lin28A antibody of the elution fractions (10% of E1) from the mock and SL-let-7g pull-downs. The relative quantification of the anti-Lin28A antibody is provided in C based on three biological replicates with the quantity of Lin28A from the SL-let-7g pull-down scaled to one and that of the mock pull-down provided as an average value with SD. In A and B, control lanes are provided that contain purified proteins ( λN⁺-L⁺-GST and Lin28A) and an NT2 WCE.