FIGURE 1.
ARiBo pull-down method for the affinity capture of RNA–protein complexes. (A) General scheme for isolating RNA-associating proteins from a WCE by RNA pull-down with an ARiBo tag. (B) Schematics of the ARiBo-tagged RNA showing the sequence and secondary structure of the ARiBo tag used in this study with annotation of the paired regions (Salvail-Lacoste et al. 2013). The ARiBo tag contains the glmS ribozyme from Bacillus anthracis, which was modified to incorporate the λBoxB RNA (gray shading) in its variable P1 stem and stabilize the P2 stem. The black arrowhead identifies the site of ribozyme-mediated cleavage.
