Structure–function relationships of archaeal Cbf5 during in vivo RNA-guided pseudouridylation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 7.
FIGURE 7.

Relevance of PUA domain and certain catalytic core residues of Cbf5 in Ψ formation in vivo. Effects of deletion of PUA domain and Ala substitution of certain conserved residues (marked above each panel) of HvCbf5 for Ψ formation at positions 1940, 1942, and 2605 were determined by primer extensions following U-specific (A,C) and CMCT reactions (B,D). The reactions were done as in Figure 3.

This Article

  1. Published in Advance August 18, 2016, doi: 10.1261/rna.057547.116 RNA 22: 1604-1619

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA