Structure–function relationships of archaeal Cbf5 during in vivo RNA-guided pseudouridylation

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FIGURE 6.
FIGURE 6.

Certain residues of the thumb loop are important for the guide RNA-dependent activity of Cbf5. Effects of alanine substitution of some conserved residues (marked above each panel) of the thumb loop of HvCbf5 for Ψ formation at positions 1940, 1942, and 2605 were determined by primer extensions following U-specific (A,C) and CMCT reactions (B,D). The reactions were done as in Figure 3.

This Article

  1. Published in Advance August 18, 2016, doi: 10.1261/rna.057547.116 RNA 22: 1604-1619

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