Structure–function relationships of archaeal Cbf5 during in vivo RNA-guided pseudouridylation

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FIGURE 5.
FIGURE 5.

Proper structure of the thumb loop is important for the activity of Cbf5. Effects of the mutations (marked above each panel) in the thumb loop of HvCbf5 on Ψ formation at positions 1940, 1942, and 2605 were determined by primer extension following U-specific (A,C) and CMCT (B,D) reactions. The reactions were done as in Figure 3.

This Article

  1. Published in Advance August 18, 2016, doi: 10.1261/rna.057547.116 RNA 22: 1604-1619

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