Structure–function relationships of archaeal Cbf5 during in vivo RNA-guided pseudouridylation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

Of the two conserved histidines of HvCbf5, H31 is required but H48 is not for rRNA Ψ formation in vivo. Effects of Ala substitution of conserved histidines (marked above each panel) of HvCbf5 for Ψ formation at positions 1940, 1942, and 2605 were determined by primer extensions following U-specific (A,C) and CMCT reactions (B,D). The reactions were done as in Figure 3.

This Article

  1. Published in Advance August 18, 2016, doi: 10.1261/rna.057547.116 RNA 22: 1604-1619

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA