Most genes reach greater than or equal to twofold change at few and disparate time points. (A) The number of time points during the 24 h collection for which each of the 1546 estrogen-responsive genes reaches a greater than or equal to twofold change from time zero. (B) The number of time points during the 24 h collection for which genes within the three classes reach a greater than or equal to twofold change from time zero. (C) The expression profile of NCOR2, a representative gene from the transient class, reaches greater than or equal to twofold change from time zero at three of the time points. (D) The expression profile of GATA3, a representative gene from the repressed class, reaches greater than or equal to twofold change from time zero at one time point. (E) The expression profile of BRIP1, a representative gene from the induced class, reaches greater than or equal to twofold change from time zero at three of the time points. All plots show the mean of three biological replicates as a blue line with box and whisker plots showing the variation in normalized expression among the replicates, and regions greater than or equal to twofold different than time zero are shaded in gray.
