CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence

CCR4 and CAF1 remove post-poly(A) sequences in an exonucleolytic manner. (A) Schematic representation of noC-A₂₀C₁G₄ and noC-UG₂₀C₁G₄. These reporter RNAs include no nucleotides with C besides the moiety of post-poly(A) sequence, whereby the only post-poly(A) sequence can be labeled by transcribing with NTP including [α-³²P] CTP. The asterisks indicate a radiolabel. (B) Deadenylation of noC-A₂₀C₁G₄ and noC-UG₂₀C₁G₄ with four types of recombinant CCR4/CAF1 heterodimer after 2 h incubation. Although CCR4 and CAF1 generated CMP from both reporter RNAs, the signal intensities from noC-A₂₀C₁G₄ in active CCR4/CAF1 heterodimers were higher than from noC-A₂₀C₁G₄ in inert CCR4/CAF1 heterodimer and from noC-UG₂₀C₁G₄. (C) Thin layer chromatography for the samples of B.