The balance between the poly(A) length and the downstream post-poly(A) length affects the activity of CCR4 and CAF1. (A) Deadenylation of noC reporter RNAs with four types of recombinant CCR4/CAF1 heterodimer after 2 h incubation. (B) Deadenylation of noC-A60C10 and noC-CG40A20C10 with four types of recombinant CCR4/CAF1 heterodimer after 2 h incubation. (C) The signal intensity of the bands in A was quantified, and the ratio of the intensity from the lower band for the total intensity was calculated and shown. As well as Figure 2C, noC-A20 effectively shifted to A0. While the length of the post-poly(A) sequence negatively correlated with the deadenylation efficiency, elongating the internal poly(A) sequence improved. The graph shows means and standard deviations (n = 3). (D) The signal intensity of the bands in B was quantified, and the ratio of the intensity from the lower band for the total intensity was calculated and shown. noC-A60C10 was deadenylated more effectively than noC-CG40A20C10. The graph shows means and standard deviations (n = 3).
