Network analysis of key altered splicing events in MDS. (A) Protein network analysis of MDS-Dx by String9.1 and Cytoscape. Line connections represent the evidence supported association. (B) Genes in the two concentrated networks are involved in splicing regulation or in cell cycle control and DNA damage repair. (C) Analysis of the effect of MDS-Dx alternative splicing (cassette exon) on the reading frame of gene transcripts. Fisher's exact test was used to compare in-/out-of-frame events of MDS-Dx, and its disease pathway-related events with overall and detectable events ([*] P < 0.05, [**] P < 0.01). For in-frame events in enriched pathways in MDS-Dx, the in/out of the protein domain was further analyzed.
