Identification of new branch points and unconventional introns in Saccharomyces cerevisiae

Lariat-set junction reads identify BP locations. (A) Schematic of lariat junction read mapping strategy. Green box indicates location of best 5′SS in lariat junction read. (B) Novel intron in BDF2 CDS is supported by Branch-seq reads (top, pink and blue as in Fig. 1) and Lariat-seq junction reads (middle, 5′SS read fragments in dark green, BP read fragments in light green). Black boxes denote novel 5′SS and BP sequences identified by Branch-seq and Lariat-seq reads. (C) Summary of overlaps among novel BPs identified by Lariat-seq JR reads, cnBP identified by Branch-seq, and novel splice junctions identified by RNA-seq.