Branch-seq locates hundreds of novel BPs. (A) Number of annotated BPs recovered by Branch-seq (light orange) compared to number of computationally predicted BPs (dark orange) (Meyer et al. 2011). The cnBPs (light green) are a subset of all novel BPs (dark green). (B) Genomic locations of the 268 cnBPs. Novel BPs located in CDS (C,D), introns (D), and of the TDA5 and RPL30 genes. Annotated TDA5 BP and 5′SS are blue. Potential AG 3′SS are depicted. 3′SS confirmed by entropy are indicated by asterisk (C,D). Potential BP-3′SS pairing indicated by matching colors (D). (E) Sequence motifs created by MEME of annotated BPs (left) and typical 5′SS cnBP (middle) recovered by Branch-seq and human BP motif (right) for comparison. Position 0 is the BP nucleotide.
