miR-200a binding may alter c-Jun ARE structure. (A) 2D prediction of the entire c-Jun 3′UTR available on UCSC (energy = −386.00 kcal/mol). miR-200b binding region is zoomed into the red box. c-Jun ARE-containing miR-200a binding site is in the green box. ARE domains and subdomains are reported and indicated alternatively in blue and yellow. (B) RNA sequence of the c-Jun ARE. miR-200a binding site is highlighted in green. (C,D) Schematic representation of the conformation of c-Jun ARE unbound (C) or bound by miR-200a (D), predicted using RNAfold algorithm. Base pair probability is reported in a color scale. Modifier letter up arrowheads (^) show UGU triplet we have changed for GUC to generate miR-200a binding site mutant (MUT-A); plus signs (+) indicate U → A mutations that do not affect mRNA stability as reported by Peng et al. (1998); asterisks (*) indicate G → C mutations that increase mRNA stability as Vlasova et al. (2008) described.
