FIGURE 7.
Mimicking the expansion of bidirectional pairings. (A) Schematic diagrams of wild-type (WT) and mutant minigene constructs to mimic expansion of the bidirectional pairing system. Mutation was performed to produce a specific enzyme site to discriminate the original exon 4.1 or 4.2 (exon 4.1* or 4.2*). (B) RT-PCR products from RNA isolated from Drosophila S2 cells transiently transfected with the insertion constructs. (C) The inclusion analysis of alternative exon 4. Quantization of the frequency of exon 4 utilization was determined by variant-specific restriction digestion. Data are expressed as percentages of the mean ± SD from three independent experiments.
