Suppression of PP2A by miR-3661 increases its target phosphorylation in human cells. (A) Schematic overview of pri-miR-3661 expression plasmids. (B) The RNA levels of Hsa-miR-3661 were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids. (C) The mRNA levels of PPP2CA and PPP2CA-iso were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids. (D) The protein levels of PP2Acα, phosphorylated AKT (p-AKT), AKT, phosphorylated GSK-3β (p-GSK-3β), and GSK-3β were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids.
