MiR-3661 is located on the opposite strand of the PPP2CA coding region in the human genome. (A) The genomic view of the PPP2CA and miR-3661 genes: coding sequence (CDS), untranslated region (UTR), and miRNA regions are represented as orange, yellow, and green bars, respectively. (AS) antisense strand, (SS) sense strand. The model for cis-antisense miRNA biosynthesis and function: the miRNA processing initiated with the splicing of pri-miRNA transcripts to yield pre-miRNA hairpins that are transported to cytoplasm. After Dicer cleavage, the mature miRNAs bind to target RNAs and direct the translation inhibition or cleavage of the sense transcripts of their targets. (B) The perfect complementary between PPP2CA and miR-3661. The binding positions are shown above (PPP2CA) and below (miR-3661) the sequences. (C) The mature miR-3661 was identified in 293FT cells using a Northern blot. The left lane was the synthetic DNA sequence of the miR-3661, which was as the positive control, and the right lane was the total RNA of 293FT cells. (D) RNA levels of PPP2CA and miR-3661 were measured from various human cell lines as indicated. (E) Scatterplots illustrating the correlation of gene expressions between PPP2CA and Hsa-miR-3661 from various human cell lines as indicated.
