Identification of cis-antisense miRNAs. (A) Flowchart of genome-wide identification of potential cis-antisense miRNAs. (B) Most of the identified cis-antisense miRNAs functionally suppress their targets in human cells. RNA levels of the cis-targeting genes (their names are listed at the top of the figure) were measured using 293FT cells transfected with the control RNA (mock mimic) and the indicated miRNAs mimic (their names are listed at the bottom of the figure) at 30 nM final concentration. Each assay was repeated in at least three independent experiments. The statistical analysis was performed by comparing the mRNA levels of target genes from the cells treated with miRNA mimic to that treated with control RNA (mock mimic). (***) P < 0.001.
