Genome-wide detection of high abundance N⁶-methyladenosine sites by microarray

m⁶A interferes with A–U or A–T pairing. (A) Agarose gel showing equal loading of 1 μg sense actB RNA containing either m⁶A or A after PAGE purification. (M) marker, (m⁶A) sense actB RNA containing only m⁶A, (A) sense actB RNA containing only A. (B) RNA protection assay (RPA) showing that m⁶A-containing RNA does not anneal to a complementary RNA as efficiently as does an A-containing probe. (P) radiolabeled probe, (P + R) radiolabeled probe digested with RNAses A and T1 for 0.5, 2, or 4 h, and (Red box) sense/antisense RNA duplex. (C) Quantification of RPA results at 4 h. (*) Student's t-test, P ≤ 0.0018, n = 2.