Increased efficiency of evolved group I intron spliceozymes by decreased side product formation

Activity and growth mediated by evolved spliceozyme mutations in E. coli cells. (A) Growth mediated by the spliceozyme variants in E. coli cells on medium containing 100 μg/mL chloramphenicol. Error bars are standard deviations from three experiments. (B) CAT enzyme activity of bacterial extract. The units correspond to 10¹² cells. Error bars are standard deviations of three experiments. Note that spliceozyme W11 corresponds to the mutations 5′3′ + U271C + helpers.