Polysome profiles of an rbfA::KmR mutant (A) and its derivatives expressing variants of r-protein S5 containing different amino acid substitutions (B–F), as well as of a wild-type strain (G). Different ribosomal particles are marked above corresponding peaks. 2x, 3x, and 4x indicate two, three, and four 70S ribosomes per mRNA, respectively. In H, the ratio is shown of the area of the peak for the 70S and 2x, 3x, and 4x polysomes to the total area of total ribosomal particles (“Associated subunits”) and the ratio of the area of the peak for the 30S subunit to the areas of the peaks for the 70S, 2x, 3x, and 4x (“30S/total associated”). The error bars represent the standard error of the mean from three independent experiments as shown for strains STN397, STN399, STN401, and STN409, and from two independent experiments for strains STN403 and STN405.
