Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor

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FIGURE 2.
FIGURE 2.

The position of the mutant amino acids of S5 (in orange) in relation to S4 (in yellow) and the central pseudoknot (helices 1 and 2; in cyan) and the neck helix (helix 28; in green) in 30S subunit (PDB ID 2AVY). The three amino acids altered in the S5 mutants described here (G87, G91, and A127) are shown in space-filling mode. The residues are located in a domain of S5 (in red) including a β sheet, composed of two β strands, and an adjoining strand. This domain is in close proximity to the central pseudoknot. The position of the spectinomycin-resistance mutant G28 is also shown in space-filling and is located near the region of h28 that is stacked on h2. The structure was modeled using VMD (Humphrey et al. 1996).

This Article

  1. Published in Advance June 18, 2015, doi: 10.1261/rna.051383.115 RNA 21: 1454-1468

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