Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor

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FIGURE 1.
FIGURE 1.

The effect of plasmid-mediated expression of the ribosome maturation proteins RimP, RimM, and RbfA on the growth of the rbfA::KmR mutant GOB162. The plates were incubated for 48 h at 30°C, 37°C, and 44°C, respectively. The plasmids used were pNTR-SD-rbfA (519#5), pNTR-SD-rimM (439#9), and pNT3-yhbC (rimP) (520#2) from the NIG collection and contain an IPTG-inducible promoter that directs the expression of the cloned genes.

This Article

  1. Published in Advance June 18, 2015, doi: 10.1261/rna.051383.115 RNA 21: 1454-1468

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