(A) Sequence of the SOLE RNA that was identified in Ghosh et al. (2012) as essential for oskar mRNA localization. The secondary structure is shown as predicted in Ghosh et al. (2012). The nucleotides in black and blue belong to the first and second exon, respectively. (PS) proximal stem; (MSL) medial stem–loop. (B) Summary of the mutant analysis performed in Ghosh et al. (2012). The four mutant sequences are shown in the boxes: Three of them support correct localization (green boxes), while one does not (dark red box). (C) Construct of the SOLE RNA used in this study together with the experimentally derived secondary structure. Two base pairs were added at the termini in comparison to the sequence of A. The new nucleotide numbering from 1 to 32 is shown with respect to the numbering of A. A continuous stacking of base pairs is seen from nucleotides 1–13 and from nucleotides 19–32. A24 is either bulged out or stacked between G23 and A25.
