NOT1 mediates interactions between CAF1 and its protein partners. (A) Schematic representation of GST pull-down assay. (B) GST-CAF1 proteins are efficiently pulled down. Western blots with antibodies to known regulatory proteins were performed after GST pull-down to test for CAF1 interaction. Western blots depict a single representative experiment, and three biological replicates were conducted. (C) Graphical representation of mass spectrometry data from three biological replicates for GST-CAF1a wild type and mutant. P-values calculated using a two-tailed Student's t-test are indicated by dot size. Members of the CCR4–NOT complex (boxed) interact strongly with CAF1a WT but not the M141R mutant. Xp54 is also enriched specifically for CAF1a WT over the M141R mutant, but to a lesser extent. (D) Same as in C, but with CAF1b.
