FIGURE 1.
(A) In vitro determined assembly map of E. coli ribosomal Small Subunit. (B) SSU assembly cascade in E. coli using TP30, in which RI is formed at low temperature and contains a subset of r-proteins. Incubation at optimal reconstitution temperature allows for the addition of the remaining r-proteins and SSU formation. (C) Assembly cascade in E. coli using individually purified r-proteins in which both RI and RI* are formed. RI and RI* are formed in the absence of late-binding r-proteins. SSUs are formed upon the addition of late-binding proteins to the RI* complex.
