Stability of the DRNT1 RNA is mediated through both DcpS and Xrn1. (A) DcpS, XRN1, and RRP41 mRNA levels were measured by qRT-PCR in HEK293T cells with control or the indicated mRNA knock down backgrounds. (B) Degradation of the indicated RNAs was determined by qRT-PCR in cells from A and as described in the legend of Figure 4. (C) Levels of DcpS and XRN1 mRNAs were measured by qRT-PCR in HEK293T cells with either a control or double DcpS and XRN1 knocking down. (D) Degradation of the DRNT1 mRNA was determined by qRT-PCR in cells from C and as described in the legend of Figure 4. All mRNA levels are presented relative to GAPDH mRNA and derived from three independent experiments; P-values from comparison of the decay rates are presented with asterisks. (***) P < 0.001 (two-tailed extra sum-of-squares F test).
