Mapping the genomic loci corresponding to HS370762 (DRNT1) and BC011766 (DRNT2) RNAs. (A) Schematic of the genomic loci and direction on Chromosome 9 for HS370762/DRNT1 is shown. Black arrows indicate position and orientation of primers used in B,C. (B) PCR of oligo(dT) reverse transcribed total RNA from HEK293T cells using the PF forward primer and the four different reverse primers (PR1, PR2, PR3, and PR4) are shown. (C) Levels of HS370762/DRNT1 RNA and potential transcript originating upstream of HS370762 quantitated by qRT-PCR from RNA derived from RG3039 treated or untreated HEK293T cells. (D) Schematic of the BC011766/DRNT2 genomic loci on chromosome 3. Black arrows indicate positions of qRT-PCR primers used in F. (E) Agarose gel of 5′RACE products. (F) Quantitation of DRNT2 and potential longer isoform of BC011766 (DRNT2) RNAs are presented relative to GAPDH mRNA and derived from three independent experiments.
