DcpS is a transcript-specific modulator of RNA in mammalian cells

RG3039 target RNAs, HS370762, and BC011766 are regulated by DcpS. (A) Endogenous and exogenously expressed DcpS protein levels were measured by Western blot in HEK293T control knock down (Con^KD) or DcpS knock down (DcpS^KD) cells, complemented with shRNA-resistant DcpS^WT of a catalytically inactive DcpS harboring a histidine 277 to asparagine (DcpS^MT) mutation as shown. Complementation with a control plasmid lacking the DcpS cDNA is denoted as “Vector.” (B) RNA levels for the indicated RNAs were measured by qRT-PCR from cells in A. RNA levels are presented relative to the GAPDH mRNA and derived from three independent experiments. RNA levels for only HS370762 and BC011766 were responsive to depletion and complementation with DcpS. Error bars represent ±SD. P values are denoted by asterisks; (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 (Student's t-test).