Validation of a subset of RG3039 target RNAs. (A) Levels of 12 randomly chosen RNAs from the 222 RNAs that deviated by at least twofold in SH-SY5Y neuroblastoma cells treated for 48 h with 100 nM RG3039 were tested by quantitative reverse-transcription (qRT)-PCR. RNA levels are presented relative to actin mRNA and derived from at least three independent experiments. Six of the RNAs were reproducibly altered greater than twofold and constitute RG3039 responsive RNAs. (B) Validated RG3039 target RNAs from SH-SY5Y cells were further confirmed in HEK293T cells to determine the consistency of RG3039 in different cell backgrounds. RNA levels are presented relative to the GAPDH mRNA and derived from at least three independent experiments. All six RNAs were similarly altered in the HEK293T cells as they were in SH-SY5Y cells. Error bars represent ±SD. (***) P < 0.001 (Student's t-test).
