Effect of incubation temperature on cell-cycle progression. (A) Human SAECs were serum-starved for 24 h at 37°C, then incubated at 32°C, 37°C, or 39.5°C for an additional 24 h in serum-containing growth medium, stained with propidium iodide, and the proportion of cells in G1, S, and G2 phases was determined using flow cytometry. (B) HEK 293T cells were transfected with a combination of synthetic miRNA inhibitors for hsa-miR-27b-5p, hsa-miR-1260a, and hsa-miR-92a-1-5p or an equal concentration of non-targeting scrambled control inhibitor. After 24 h recovery, the cells were serum-starved for 24 h at 37°C, then incubated in serum-containing growth medium at 32°C or 37°C for 24 h, and cell-cycle analysis performed and the G1:S ratio calculated. Mean ± SEM, n = 5, (*) P < 0.05 in A. P-values are indicated in B.
