FIGURE 3.
Quantitative RT-PCR analysis of temperature effects on pri-miRNA, pre-miRNA, and opposite strand expression pattern in human SAECs. Human SAECs from two lots were incubated for 24 h at 32°C, 37°C, or 39.5°C without rhTNFα. Total RNA was collected and levels of the temperature-responsive strand, the sister strand, the pri-miRNA, and the pre-miRNA for (A) hsa-miR-92a-1, (B) hsa-miR-27b, (C) hsa-miR-27a, and (D) hsa-miR-181a were analyzed by qRT-PCR as described in Materials and Methods and are expressed relative to expression of each in 37°C hSEAECs. Mean ± SEM, n = 4. The P-values from one-way ANOVA are indicated.
