Shifts in temperature within the physiologic range modify strand-specific expression of select human microRNAs

Microarray and nCounter analysis of the effects of incubation temperature on miRNA expression pattern in human SAECs. (A) HEATMAP of selected miRNAs detected by microarray in hSAECs following 24 h incubation at 32°C, 37°C, or 39.5°C in the absence or presence of 1 ng/mL rhTNFα. (B,C) Microarray data for seven miRNAs exhibiting temperature-dependent expression and nCounter data for miR1260a expression expressed as fold-change versus 37°C cells in the absence (B) or presence (C) of TNFα. Mean ± SEM. (*) P < 0.01 versus 37°C. N = 3 for microarray and 4 for nCounter.