FIGURE 1.
(A) Secondary structures of CRZ-2 and PBD 1–4 shown in one of the two possible conformations (cf. Scheme 2). Sequence changes in comparison to the reference RNA CRZ-2 are shown in red. (B) Sequence alignment of the four designed RNAs PBD1–4 with the reference system CRZ-2. Green interior loop areas are reported to be essential for cleavage/ligation activity and were therefore fixed during the design process. The orange colored T7 RNA promoter sequence was needed for experimental implementation. The secondary structure in dot-bracket notation below shows the constraints on a structural level.
