FIGURE 2.
Stimulation of GTPase activity of YjeQ variants by mature and immature 30S subunits. The GTP hydrolysis rate of the YjeQ variants alone or in the presence of mature (Mt 30S) and immature 30S (Im 30S) subunits was assessed using the Malachite Green Phosphate assay as described in Materials and Methods. The GTPase hydrolysis rates plotted in the graph were determined by measuring the free phosphate produced after the reactions had been incubated for 60 min at 25°C. Standard deviations shown in the plot correspond to three replicas of the experiment.
