Modulation of Ox-LDL uptake by OLR1 AS regulation. (A) Schematic representation of targeting sites for AONs used to modulate OLR1 AS. SNP corresponds to an AON complementary to the region if intron 4 containing rs3736234 in its Low-Risk configuration. (B) Modulation of OLR1 AS by AONs. Exon 5 percent spliced in (PSI) values for endogenous OLR1 transcripts from HeLa cells upon transfection of the indicated AONs, measured by capillary electrophoresis, are indicated. Values represent mean and standard deviation of nine independent biological experiments. (C) RT-qPCR quantification of expression of exon 5 skipping isoform (LOXIN) normalized to HPRT1 mRNA expression upon transfection of the indicated AONs or SRSF expression vectors. Values represent mean and standard deviation of three independent biological experiments. (D) Measurement of Ox-LDL uptake under the same conditions as in C, monitored by FACS analysis quantification of Dil-Ox-LDL. Values correspond to mean and standard deviation for three independent experiments. P-values of results in this figure correspond to Student's two-tailed heteroscedastic t-test: (*) <0.05, (**) <0.01, (***) <0.001.
