Effects of SRSF1/SRSF2 overexpression on OLR1 AS regulation upon deletion of their putative binding sites. (A) Schematic representation of wild type and mutant minigenes used in this experiment. Nomenclature and symbols as in Figure 5A. (B–E) Splicing changes induced in the indicated minigenes by overexpression of the indicated proteins, analyzed as in previous figures. Numbers below indicate nanogram of protein expression vector co-transfected in each condition, with the total amount of transfected plasmid adjusted to 1000 ng using pCMV empty vector. For all the panels, data represent mean and standard deviations of inclusion/skipping ratio for eight independent experiments. P-values obtained from Student's two-tailed heteroscedastic t-test are indicated: (**) <0.01, (***) <0.001.
