Additional nucleotide identities in the allelic series attenuate the effect of SNP rs3736234 on OLR1 AS. (A) Nucleotide-specific effect of rs3736234 and rs13306593 SNP substitutions. The scheme indicates the individual substitutions from Low- to High-Risk allelic series and from Low to a different nucleotide. Data represent mean and standard deviation of exon 5 inclusion/skipping ratios of the different constructs after normalization to Low-Risk minigenes for six independent biological replicas. Significant P-values obtained from Student's two-tailed heteroscedastic t-test are indicated: (*) <0.05, (**) <0.01. (B) The table indicates the accumulative series mutations introduced in the Low-Risk minigene to reconstruct the genotype of the High-Risk allele series (in bold). (C) Ratio of exon 5 inclusion/skipping for the constructs indicated in B, normalized to the values of the Low-Risk minigene. Values represent mean and standard deviation for eight independent biological replicas. P-values for each of the conditions are indicated.
