FIGURE 8.
Genetic interactions of Snp1 amino-terminal truncations. (Top) Alignment of the amino-terminal amino acid sequences of the S. cerevisiae Snp1 and human U1-70K. Positions of side chain identity/similarity are indicated by • above the alignment. Arrowheads indicate the boundaries of the ▵N truncations of Snp1. (Bottom) Yeast snp1▵ strains bearing the indicated SNP1-▵N allele on a CEN HIS3 plasmid in an otherwise wild-type (top panel), mud2▵, mud1▵, nam8▵, or tgs1▵ background as indicated were spot-tested for growth on YPD agar at the temperatures specified. Synthetic growth defects are denoted by •.
